Development of an SNP Marker for Sugar Beet Resistance/Susceptible Genotyping to Root-Knot Nematode

Linked and/or gene-based molecular markers have been used widely in marker-assisted selection (MAS) to differentiate resistant and susceptible genotypes. Resistance to Meloidogyne spp. in Beta vulgaris L. is mediated by a single dominant gene (R۶m-۱). Using allele-specific primers (ASPs), an SNP marker harboring a single nucleotide polymorphism (A/G), linked to the resistance gene was developed to differentiate resistant genotypes. The differentiation among the resistant and susceptible genotypes was elucidated in the polymorphic bands of ۵۵۵, ۴۷۸ and ۱۲۴ bp in size, using PCR amplification. The genotyping data using the SNP marker was firmly associated with the bioassay evaluation in the greenhouse for ۱۰۰ sugar beet genotypes. This data indicated that the present robust marker allowed reliable, sensitive, faster, and cheaper large scale screening of B. vulgaris genotypes for nematode resistance breeding programs.

Keywords:

Beta vulgaris , Meloidogyne spp , Selection , SNP marker