An Investigation into the Effects of Cadmium and Nitric Oxide on Induced Secondary Metabolism and Antioxidant System in Pimpinella anisum L. Through Transcriptional Upregulations in AIS۱, PAL, SOD, R۲R۳-MYB, and bZIP Genes

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 47

This Paper With 7 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_JJMPB-11-1_015

تاریخ نمایه سازی: 6 آذر 1402

Abstract:

This study was aimed to evaluate the cytoprotective role of nitric oxide (NO) against cadmium (Cd) through the potential transcriptional modifications in the expression of genes. Pimpinella anisum seedlings were treated with Cd (۰ and ۱ mM) and/or NO (۰ and ۲۵ µM). The Cd-associated decreases in shoot and root biomass were mitigated by the exogenously applied NO. Proline concentrations were increased in response to NO and/or Cd. Cd and NO treatments caused a considerable increase in peroxidase activities in comparison to the control. The individual Cd treatment led to the slight significant (p≤۰.۰۰۱) up-regulation in expression of R۲R۳-MYB transcription factor by ۲.۵ folds, while the simultaneous exposure to NO and Cd stimulated this gene by ۷.۲ folds (p≤۰.۰۰۰۱). With a similar trend, NO significantly (p≤۰.۰۰۰۱) upregulated bZIP transcription factor by ۵.۹ folds in Cd-treated seedlings.  Likewise, upregulation (۵.۲ folds) in the significant (p≤۰.۰۰۰۱) increase in expression of the phenylalanine ammonia-lyase (PAL) gene resulted from the NO+Cd application. Moreover, t-anol/isoeugenol synthase ۱ (AIS۱) gene was slightly stimulated (p≤۰.۰۵) in response to individual NO treatment. However, the Cd treatment resulted in a drastic significant (p≤۰.۰۰۰۱) increase in AIS۱ expression by ۴۲ folds which was partly declined in the Cd+NO group and reached ۲۲ folds (p≤۰.۰۰۰۱). Moreover, the NO+Cd treatment caused significant (p≤۰.۰۰۰۱) induction in the transcription of the superoxide dismutase (SOD) gene by ۱۱.۳ folds.  The correlation observed among expression patterns of mentioned genes, therefore, highlights the close interplay between the transcription factors, secondary metabolism, and antioxidant system.

Authors

Sepideh Hajbagheri

Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Hossein Abbaspour

Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran

Shokoufeh Enteshari

Department of Biology, Payame Noor University, Tehran, Iran

Saeed Habibollahi

Department of Biology, Payame Noor University, Tehran, Iran

Alireza Iranbakhsh

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Safari M, Ardebili Z.O, Iranbakhsh A. Selenium nano-particle induced alterations ...
  • Ali S, Rizwan M, Noureen S, Anwar S, Ali B, ...
  • Babajani A, Iranbakhsh A, Ardebili Z.O, Eslami B. Seed priming ...
  • Shanmugaraj BM, Malla A, Ramalingam S. Cadmium stress and toxicity ...
  • Singh S, Prasad SM, Sharma S, Dubey NK, Ramawat N, ...
  • Corpas FJ. Nitric Oxide and Hydrogen Sulfide in Higher Plants ...
  • Groß F, Durner J, Gaupels F. Nitric oxide, antioxidants and ...
  • Nazerieh H, Aredebili ZO, Iranbakhsh A. Potential benefits and toxicity ...
  • Iranbakhsh A, Ardebili ZO, Molaei H, Ardebili O, Amini M. ...
  • Shams M, Ekinci M, Ors S, Turan M, Agar G, ...
  • Kaya C, Okant M, Ugurlar F, Alyemeni M.N, Ashraf M, ...
  • Santisree P, Bhatnagar-Mathur P, Sharma K.K. Molecular insights into the ...
  • Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, ...
  • Inne R. The positives and negatives of NPR, a unifying ...
  • Khan M.N, Siddiqui M.H, Mohammad F, Naeem M. Interactive role ...
  • Sun W, Shahrajabian MH, Cheng Q. Anise (Pimpinella anisum L.), ...
  • Ahmad P, Ahanger M.A, Alyemeni M.N, Wijaya L, Alam P. ...
  • Dong Y.J, Chen W.F, Liu F.Z, Wan Y.S. Physiological responses ...
  • Mostofa MG, Seraj Z.I, Fujita M. Exogenous sodium nitroprusside and ...
  • Basalah M.O, Ali H.M, Al-Whaibi M.H, Siddiqu M.H, Sakran A.M, ...
  • Zha H, Jin Q, Wang Y, Chu L, L X, ...
  • Laspina NV, Groppa MD, Tomaro L, Benavides MP. Nitric oxide ...
  • Xu J, Wang W, Yin H, Liu X, Sun H, ...
  • John R, Ahmad P, Gadgil K, Sharm S. Cadmium and ...
  • Melo L.C, Alleoni L.R, Carvalho G, Azevedo RA. Cadmium‐and barium‐toxicity ...
  • Shan S, Liu F, Li C. Effects of cadmium on ...
  • Singh A, Prasad S.M. Effect of agro-industrial waste amendment on ...
  • He J, Ren Y, Chen X, Che H. Protective roles ...
  • Wang Q, Liang X, Dong Y, Xu L, Zhang X, ...
  • Stracke R, Werber M, Weisshaar B. The R۲R۳-MYB gene family ...
  • Cao Y, Han Y, Li D, Lin Y, Cai Y. ...
  • Chen C, Zhang K, Khurshid M, Li J, He M, ...
  • Lv Z, Guo Z, Zhang L, Zhang F, Jiang W, ...
  • Han J, Liu H.T, Wang S.C, Wang C.R, Miao G.P. ...
  • نمایش کامل مراجع