Secondary Metabolite Contents and Antioxidant Enzyme Activities of Cichorium intybus Hairy Roots in Response to Zinc
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JJMPB-2-2_003
تاریخ نمایه سازی: 6 آذر 1402
Abstract:
Hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” Agrobacterium rhizogenes. In most plants such as Cichorium intybus L., hairy root cultures have proven to be an efficient system for secondary metabolites production. The effect of Zinc (ZnSO۴), a heavy metal, was investigated at different concentrations (۰, ۱, ۵ and ۱۰ mM) on some secondary metabolite contents at three time course levels (۲۴, ۴۸ and ۷۲ h).The treated hairy roots of chicory were compared with control and with each other in growth rate, phenol flavonoid and chicoric acid production rate. In addition, antioxidant enzyme activities were determined. Results showed decreased hairy roots weights and increased phenol, flavonoid, chicoric acid and antioxidant enzyme activities in response to higher concentrations of Zinc at higher time courses. Also, an increase in chicoric acid release into the culture media was observed that is important for industrial uses.
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Authors
Bentolhoda Azarmehr
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Farah Karimi
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Masoud Taghizadeh
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Seyed Latif Mousavi Gargari
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
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