Growth Inhibitory Effect of Anthemis haussknechtii Root Extract, as a Source of Parthenolide, on Breast Cancer Cell Line

Parthenolide is major Sesquiterpene lactones present in Anthemis haussknechtii Boiss. & Reut. (feverfew). This compound has many effects on different disease such as migrain and cancer. Parthenolide was reported from Tanacetum parthenium (L.) Sch.Bip. but other plants of Asteraceae family could contain parthenolide. In this study parthenolide was extracted and identified with two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Breast cancer cell line, MDA-MB-۲۳۱, was exposed to different concentrations of parthenolide for ۲۴ hours.  Half maximal inhibitory concentration (IC۵۰) was evaluated using Methylthiazol Tetrazolium (MTT) test. Based on results, ۱۰۰۰ µg/ml concentration is the minimum lethal dose that kills approximately ۵۰% of cells after ۲۴ hours. The results revealed that the A. haussknechtii parthenolide dramatically decreased survival of cancer cell line by inducing apoptosis. This is the first report of cytotoxicity effect of A. haussknechtii extract on breast cancer cell line.

Keywords:

Cell Viability , FT-IR , HPLC , MTT test , Parthenolide