Antifungal activity of Gracilaria corticata methanol extract against Trichophyton mentagrophytes, Microsporum canis, and Microsporum gypseum on rat dermatophytosis models
Publish place: Current Medical Mycology، Vol: 9، Issue: 1
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_CUMM-9-1_003
تاریخ نمایه سازی: 9 آذر 1402
Abstract:
Background and Purpose: Dermatophytosis is one of the most prevalent zoonotic diseases. Increased resistance of dermatophytosis causing pathogens against antidermatophytic agents highlights the need for alternative medicine with higher efficiency and lower side effects. In the present study, the in vitro antifungal activities of different concentrations of Gracilaria corticata methanol extract against Trichophyton mentagrophytes, Microsporum canis, and Microsporum gypseum were assessed and their efficacy was evaluated in rat dermatophytosis models.Materials and Methods: The broth microdilution and well diffusion methods were used to determine the in vitro antidermatophytic activity. The in vivo study was carried out using ۴۰ dermatophytosis-infected adults male Wistar rats. The animals were divided into ۴ groups (۵% and ۱۰% G. corticata ointment, terbinafine, and Vaseline) and treated with ointment until complete recovery. The percentage of wound closure was calculated for each group.Results: The results revealed that G. corticata methanol extract was effective to varying extents against the tested dermatophytes. The highest inhibitory activity of G. corticata was found against T. mentagrophytes with minimum inhibitory concentration and minimum fungicidal concentration values of ۴ and ۹ µg mL-۱, respectively. The in vivo experiment revealed that ۱۰% G. corticata ointment significantly accelerated skin lesions reduction and completely cured M. gypseum, T. mentagrophytes, and M. canis infections after ۱۹, ۲۵, and ۳۸ days, respectively.Conclusion: The methanol extract of G. corticata exhibited significant antifungal activity in vitro and in vivo, suggesting that it could be used as an alternative to antidermatophytic therapy in a dose-dependent manner.
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Authors
Asiyeh Shojaee
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Alireza jahandideh
Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran
Ayatollah Nasrollahi Omran
Department of Mycology, Faculty of Medical sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Nakisa Sohrabi Haghdoost
Department of Pathobiology, School of Veterinary science and Research Branch, Islamic Azad University, Tehran, Iran
Mehrzad khosravi
Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran
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