Effects of thiamine on growth, aflatoxin production, and aflr gene expression in A. parasiticus

Background and Purpose: Mycotoxins are secondary fungal metabolites with a very high diversity that are produced by some species of Aspergillus which frequently leads to contaminate food and agricultural products. Recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide. Although, the antibacterial and antifungal effects of vitamins as natural compounds have been proven, the mechanism of vitamins effect on Aspergillus parasiticus growth and aflatoxin production is not yet clear. In this study, the effect of thiamine (vitamin B۱) was studied on Aspergillus parasiticus growth, aflatoxins production and the afIR gene expression. Materials and Methods: A standard strain of Aspergillus parasiticus was applied for performing antifungal susceptibility test in different concentrations of thiamine. Antifungal susceptibility test was performed according to CLSI M۳۸-A۲ document. The concentration of aflatoxin was determined by HPLC. Moreover, the quantitative changes in the aflR gene expression were analyzed by Real Time PCR method. Results: The minimum inhibitory concentration was yielded as > ۵۰۰ mg/ml. However, HPLC analysis results showed that aflatoxin production reduced in samples treated with ۵۰۰ mg/ml of thiamine. In addition, the level of afIR gene expression was significantly reduced after treating with ۵۰۰ and ۲۵۰ mg/ml of vitamin B۱. Conclusion: Based on the obtained results, thiamine could not inhibit the fungal growth completely. However, the rate of afIR gene expression and aflatoxin production was significantly reduced after fungal treating with thiamine. Consequently, using natural compounds such as vitamins may be regarded as potential antitoxic agent in food industry and the industries related to agriculture.

Keywords:

Aflatoxins , Aspergillus , AFLR protein , Gene expression , Thiamine