Synergistic Effects of Silver Nanoparticles with Ethanolic Extract of Eucalyptus globules on Standard Pathogenic Bacteria in Vitro
Publish place: Tabari Biomedical Student Research Journal، Vol: 2، Issue: 3
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_TBSRJ-2-3_003
تاریخ نمایه سازی: 11 آذر 1402
Abstract:
Introduction: Nowadays, with the increase of resistance due to overuse of synthetic chemical antibiotics, it seems necessary to find alternative drugs. The aim of this study was to compare the effects of silver nanoparticles and Eucalyptus globules (eucalyptus) ethanolic extract on standard bacteria of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli.
Material and Methods: In this experimental study, aerial parts of Eucalyptus plant were collected from Marand city and identified as Eucalyptus plant by botanists of Islamic Azad University, Ahar Branch. In this study, eucalyptus ethanolic extract was prepared by Soxhlet method and the antibacterial effects of eucalyptus extract at concentrations of ۲۰, ۳۰, ۵۰ and ۴۰۰ mg / ml and silver nanoparticles at concentrations of ۱۰, ۲۰, ۴۰ and ۸۰ μg / ml with agar well diffusion methods and tubular dilution were investigated.
Results: The results showed that the ethanolic extract of Eucalyptus had more antibacterial properties compared to silver nanoparticles. Eucalyptus extract and silver nanoparticles had a greater effect on gram-positive bacteria. The effect of the combination of eucalyptus extract and silver nanoparticles was much greater than the effect of either.
Conclusion: The results showed that silver nanoparticles in combination with eucalyptus extract have good antimicrobial activity against pathogenic bacteria. Therefore, this extract along with silver nanoparticles can be a good option for future studies in vivo to prepare antibacterial drugs.
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Authors
Abolfazl Jafari Sales
Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
Afsoon Shariat
Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
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