A Histochemical Comparison of Feulgen and Papanicolaou Stains in Demonstrating Cytotoxic and Genotoxic Effects of Cigarette Smoking on Human Buccal Mucosa Cells
Publish place: Avicenna Journal of Dental Research، Vol: 15، Issue: 2
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_AJDR-15-2_001
تاریخ نمایه سازی: 14 آذر 1402
Abstract:
Background: Different histochemical stains have been applied to demonstrate the cytotoxic and
genotoxic effects of cigarette smoking on cells. Feulgen and Papanicolaou were the most popular stains to
demonstrate nuclear abnormalities. The aim of this study was to compare Feulgen and Papanicolaou stains
in demonstrating the cytotoxic and genotoxic effects of cigarette smoking on exfoliated oral mucosa cells.
Methods: A total of ۳۱ cigarette smokers and ۱۵ non-smokers were included in this case-control study.
Using a wooden spatula, two samples were taken from each participant. The samples from the left
buccal mucosa were stained with Feulgen and the right mucosa with Papanicolaou. The mean number
of micronuclei and the number of cells with pyknosis, karyorrhexis, and karyolysis were determined on
Feulgen and Papanicolaou-stained slides. The number of counted cells with pyknosis, karyorrhexis, and
karyolysis in ۱۰۰۰ cells/subject was recorded. The mean number of micronuclei was determined by the
number of counted micronuclei per ۱۰۰۰ cells per subject.
Results: The number of micronuclei was not significantly different between Feulgen and Papanicolaou
stained samples (P = ۰.۲۷). Demonstration of karyolysis (P = ۰.۷۳) and karyorrhexis (P = ۰.۲۴) was not
significantly different between Feulgen and Papanicolaou staining methods. The Feulgen was significantly
more effective in demonstrating pyknosis compared to Papanicolaou (P = ۰.۰۲).
Conclusions: Feulgen and Papanicolaou stains had similar effectiveness in demonstrating DNA alterations
(micronucleus) and cellular death features (karyorrhexis and karyolysis). Feulgen was preferable to display
pyknosis than Papanicolaou.
Keywords:
Authors
Iman Yarmohammadi
Graduated from Faculty of Dentistry, Shahed University, Tehran, Iran
Noushin Jalayer Naderi
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University, Tehran, Iran