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Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine

Publish place: Archives of Razi Institute journal، Vol: 71، Issue: 1
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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https://civilica.com/doc/1868777

شناسه ملی سند علمی:

JR_ARCHRAZI-71-1_002

تاریخ نمایه سازی: 6 دی 1402

Abstract:

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at ۷ and ۲۸ days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-γ, TNF-α and IL-۱۰ expression using relative real-time PCR assay. Our results showed that all of the genes wereupregulated. The expression of TNF-α and IL-۱۰ genes significantly increased (P<۰.۰۵) in day ۲۸th incomparison to the day ۷th post the first vaccination. It can be concluded that the vaccine induced immuneresponses by increasing expression of the cytokines. Therefore, effects of DNA vaccines on immune system alsomay be evaluated using these genes.

Keywords:

Cytokine , mRNA expression , FMD , Guinea pig , Real-time PCR

Authors

R. Pasandideh

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

M.R. Nassiri

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

M. Raouf Delgosha

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

A.A. Aslaminejad

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

M. Tahmoorespur

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

S. Zibaei

Razi vaccine and Serum Research Institute, Mashhad, Iran

M. Doosti

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

M. Pasandideh

Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

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