Simple and sensitive high performance liquid chromatographic method for the simultaneous quantitation of the phenylalanine in human plasma

Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_PBRE-1-3_002

تاریخ نمایه سازی: 10 دی 1402

Abstract:

Phenylalanine (Phe) is the most reliable indicator for the diagnosis of phenylketonuria (PKU). The purpose of this study is to establish a reliable and quick method for the assignment of Phe in peripheral capillary blood from newborns and children by high performance liquid chromatography with ultraviolet detection (HPLC-UV). PKU is an inborn error of metabolism characterized by the inability of the body to use Phe. A rapid and sensitive high performance liquid chromatographic (HPLC) method has been developed for determination of Phe in plasma. The method uses a protein precipitation step with sulfosalicilic acid for sample preparation by separation on a Nova-pack C۱۸ column using sodium acetate buffer and acetonitrile (۹۴: ۶ v/v) adjusted to pH ۶.۵ with glacial acetic acid. The eluted peaks detected by a UV detector was set at wavelength of ۲۱۵ nm. The method was validated in the range of Phe concentrations from ۰.۱ to ۲۰ µg/ml. The limits of detection (LOD) and quantitation (LOQ) of the method were ۰.۰۵ and ۰.۱ µg/ml, respectively. The average drug recovery from plasma was ۸۸.۶۰ percent throughout the linear concentration range., with the average within-run and between-run accuracy values of ۱۰۳.۳ and ۱۱۵.۳۵۰, respectively. The method is quick, easy, very steady and precise for the screen, assignment, and evaluation of Phe in human plasma by HPLC, which is particularly a useful way for screening and diagnosis of PKU and monitoring of a diet therapy.

Authors

Hossein Danafar

Department of Medicinal Chemistry, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran

Mehrdad Hamidi

Department of Pharmaceutics, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran

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  • Belmont-Martínez L, Fernández-Lainez C, Ibarra-González I, Guillén-López S, Monroy- Santoyo ...
  • Mitchell JJ. Phenylalanine Hydroxylase Deficiency. In: Pagon RA, Adam MP, ...
  • Scriver C, Kaufman S. The hyperphenylalaninemias. In: Scriver C, Beaudet ...
  • Smith I, Philip L. The hyperphenylalaninemias. In: Fernandes J, Saudubray ...
  • Guthrie R, Susi A. A simple phenylalanine method for detecting ...
  • Levy PA, Miller JB, Shapira E. The advantage of phenylalanine ...
  • Eastman JW, Sherwin JE,Wong R, Liao CL, Currier RJ, Lorey ...
  • Hilton MA. Liquid-chromatographic direct determination of phenylalanine andtyrosine in serum ...
  • Roesel AR, Blankenship PR, Hommes F. HPLC assay of phenylalanine ...
  • Rudy JL, Rutledge JC, Lewis SL. Phenylalanine and tyrosine in ...
  • Atherton ND, Green A. HPLC measurement of phenylalanine in plasma. ...
  • Vollmer DW, Jinks DC, Guthrie R. Isocratic reverse-phase liquid chromatographyassay ...
  • Qu Y, Miller JB, Slocum RH, Shapira E. Rapid automated ...
  • Reilly AA, Bellisario R, Pass KA. Multivariate discrimination for phenylketonuria(PKU) ...
  • Allen KR, Degg TJ, Rushworth PA, Smith M, Henderson MJ. ...
  • Dale Y, Mackey V, Mushi R, Nyanda A, Maleque M, ...
  • Hong M, Tang AG. Determination of phenylalanine and tyrosine in ...
  • Jeong JS, SimHJ, Lee YM, Yoon HR, Lee DH, Hong ...
  • Kand'ár R, Záková P. Determination of phenylalanine and tyrosine in ...
  • Kim NH, Jeong JS, Kwon HJ, Lee YM, Yoon HR, ...
  • Madira WM, Xavier F, Stern J, Wilcox AH, Barron JL. ...
  • Kok WT, Brinkman UA, Frei RW. Rapid determination of phenylalanine ...
  • Qu Y, Slocum R, Fu J, Rasmussen WE, Rector HD, ...
  • Bartolomeo MP, Maisano F. Validation of a reversed-phase HPLC method ...
  • Gatti R, Gioia MG. Liquid chromatographic fluorescence determination of amino ...
  • Kandar R, Zakova P. Determination of phenylalanine and tyrosine in ...
  • Gerasimova NS, Steklova IV, Tuuminen T. Fluorometric method for phenylalanine ...
  • Wendel U, Koppelkamm M, Hummel W, Sander J, Ulrich L. ...
  • Wibrand F. Amicroplate-based enzymatic assay for the simultaneous determination of ...
  • De Silva V, Oldham CD, May SW. L-Phenylalanine concentration in ...
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