Evaluation of G۲۶۷۷T/A polymorphism of MDR۱ gene by polymerase chain reaction in Mazandaran province, Iran

The human MDR۱ gene encodes for a P-glycoprotein (PGP), which acts as an efflux pump that transports a large variety of substrates from the inside of cells to the outside until protection against xenobiotics. The G۲۶۷۷T/A polymorphism in exon ۲۱ is associated with PGP expression and function in humans. The present study was aimed to determine the frequencies of this polymorphism in a healthy population from Mazandaran province of Iran. A total of ۱۲۰ unrelated healthy subjects from Mazandaran province, residing in Sari, coming for blood donating at Sari Blood Transfusion Center were enrolled. Genomic DNA was extracted from the peripheral blood lymphocytes of each subject. All subjects were genotyped for G۲۶۷۷T/A polymorphism by polymerase chain reaction-restriction fragment length polymorphism method. The genotype frequencies were G۲۶۷۷G (۶۵%), G۲۶۷۷T (۲۰.۸۳%), G۲۶۷۷A (۱۴.۱۷%) and TT, AA, TA genotypes were not observed. Moreover, frequency of G allele (۸۲.۵%) was significantly (p ˂ ۰.۰۵) higher than the T (۱۰.۴۲%) and A (۷.۰۸%). This is the first study to investigate the G۲۶۷۷T/A polymorphism of MDR۱ gene in population from Mazandaran province of Iran. These data may be relevant for dose recommendation of PGP substrate drugs and can help for individualizing drug therapy of organ transplantation and important diseases such as cancer and AIDS, congestive heart failure and etc.

Keywords:

Naproxen , release rate , hot melt method , solid dispersion