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Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria

Publish Year: 1399
Type: Journal paper
Language: English
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JR_REMJ-8-4_005

Index date: 10 January 2024

Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria abstract

Background: Probiotics are “live microbial cells” that are beneficial for human and animal health. Lactobacilli are such a diverse group of bacteria with similar metabolic and physiological characteristics, and constitute important and beneficial gut microflora. During carbohydrate fermentation, lactobacilli produce lactic acid as an end product in metabolism. Hence, lactobacilli have high significance to be used as probiotics in the food industry, because of their acidifying properties. Also, lactobacilli are considered “safe”, owing to their ubiquitous presence in the food. Many researchers provided evidence for the presence of lactobacilli in milk sources. Thus, the present study aimed to isolate and characterize different lactobacilli strains from milk sources and analyze their “probiotic potential”. Materials and methods: Forty-one lactobacilli isolates were obtained from raw cow milk. Then, the strains were characterized by morphological identification and biochemical tests. Besides, probiotic potentials were evaluated with the bile tolerance test, antibiotic susceptibility test, and determining suitable pH for the optimal growth of lactobacilli. The lactobacilli isolates were also analyzed for their probiotic characteristics and the release of antimicrobial substances. Their antimicrobial activities against pathogenic strains were assessed by determining the minimum inhibitory concentration, with the help of agar diffusion methods. Results: From 50 milk samples, 41 lactobacilli isolates were obtained, out of which five lactobacilli strains were identified as Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus acidophilus, and Lactobacillus lactis. Moreover, 35 isolates showed an inhibitory effect. These strains were able to survive and grow in 0.5% to 2.5% bile salt concentrations. Lactic acid bacteria were susceptible to antibiotics, and 35 isolates obtained from raw milk showed an inhibitory effect against pathogenic bacteria. The observed minimum inhibitory concentration ranged from 50 to 100 µL and varied between the different pathogens. Conclusion: Out of 41 Lactobacillus isolates obtained from cow milk samples, 35 were identified with probiotic characteristics. Hence, this study highlighted the novel probiotic bacteria and validated the antimicrobial properties of the Lactobacillus spp against pathogenic bacteria.

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Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria authors

Ciamak Ghazaei

Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran.

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Mohr KI. History of Antibiotics Research. In: Stadler M, Dersch ...
Bechinger B, Gorr S-U. Antimicrobial Peptides: Mechanisms of Action and ...
Munita JM, Arias CA. Mechanisms of Antibiotic Resistance. In: Kudva ...
Baharoglu Z, Mazel D. SOS, the formidable strategy of bacteria ...
Joint FAO/WHO Expert Consultation on. ۲۰۰۱; ۱-۳۴ ...
Rashid S, Hassanshahian M. Screening, Isolation and Identification of Lactic ...
Both E, György É, Kibédi-Szabó CZ, Tamás É, Ábrahám B, ...
Saarela M, Mogensen G, Fondén R, Mättö J, Mattila-Sandholm T. ...
Marteau P, Minekus M, Havenaar R, Huis In’t Veld JHJ. ...
Shewale RN, Sawale PD, Khedkar C, Singh A. Selection Criteria ...
Ouwehand AC, Salminen S, Isolauri E. Probiotics: an overview of ...
Niazi Amraii H, Abtahi H, Jafari P, Mohajerani HR, Fakhroleslam ...
Prabhurajeshwar C, Chandrakanth K. Evaluation of antimicrobial properties and their ...
Buzby JC, Roberts T., Lin CT, MacDonald JM. Bacterial Foodborne ...
Tebyanian H, Bakhtiari A, Karami A, Kariminik A. Antimicrobial Activity ...
Wang Q, Yamabe K, Narita J, et al. Suppression of ...
Yang YJ, Chuang CC, Yang HB, et al. Lactobacillus acidophilus ...
Hartemink R, Domenech VR, Rombouts FM. LAMVAB e a new ...
Bauer AW, Kirby WMN, Sherries JL. Antibiotics susceptibility testing a ...
Brenner DJ, Staley JT, Krieg NR. Classification of procaryotic organisms ...
Hossein N, Stenzel DJ, Britz ML. Effect of growth at ...
Gilliland SE, Staley TE, Bush LJ. Importance of bile tolerance ...
ISO (International Organization for Standardization). Milk and milk products-determination of ...
Prabhurajeshwar C, Chandrakanth RK. Probiotic potential of Lactobacilli with antagonistic ...
Mami AZ, Boumehira AR, Hamedi JE, Henni M. Screening of ...
Toure R, Kheadr E, Lacroix C, Moroni O, Fliss I. ...
Martinez JL. General principles of antibiotic resistance in bacteria. Drug ...
Wassie M and Wassie T. Isolation and Identification of Lactic ...
Elizete DFRP and Carlos RS. Biochemical characterization and identification of ...
Vanniyasingam J, Kapilan R, Vasntharuba S. Isolation and characterization of ...
Azat R, Liu Y, Li W, Kayir A, Lin D, ...
Sanders ME. Considerations for use of probiotic bacteria to modulate ...
Hiromi K, Masaru N, Miho K, Takashi O, Sadahiro O. ...
Shivram PL, Vishwnath PP. Assessment of probiotic potential of Lactobacillus ...
Fallingborg J. Intraluminal pH of the human gastrointestinal tract. Dan ...
Evans DF, Pye G, Bramley R, Clark AG, et al. ...
Fernandez MF, Boris S, Barbes C. Probiotic properties of human ...
Dunne C, O’ Mahony L, Murphy L, Thornton G, et ...
Soon, J, Chadd S, and Baines R. Escherichia coli O۱۵۷: ...
Hassan M, Kjos M, Nes I, et al. Natural antimicrobial ...
Palsboll, P.J., J. Allen, M. Berube, et al. Genetic tagging ...
Sirsat S, Muthaiyan A, and Ricke S. Antimicrobials for foodborne ...
Mani-López E, García HS, and López-Malo A. Organic acids as ...
Newell DG, Koopmans M, Verhoef L, et al. Food-borne diseases—The ...
Laxminarayan R, Duse A, Wattal C, et al. Antibiotic resistance—The ...
Liu G, Pilla G, Tang CM. Shigella host: pathogen interactions: ...
Li H, Xu C-X, Gong R-J, Chi J-S, Liu P, ...
World Gastroenterology Organisation Global Guidelines: Probiotics and Prebiotics. ۲۰۱۱. https://insights.ovid.com/crossref?an=۰۰۰۰۴۸۳۶-۲۰۱۲۰۷۰۰۰-۰۰۰۰۹ ...
Nighswonger BD, Brashears MM, Gilliland SE. Viability of Lactobacillus acidophilus ...
Osuntoki AA, Ejide OR, Omonigbehin EA. Antagonistic effects on enteropathogenic ...
Alakomi HL, Skytta E, Saarela M, Mattila-Sandholm T, et al. ...
Davoodabadi A, Soltan Dallal MM, Lashani E, Tajabadi Ebrahimi M. ...
McFarland LV. Probiotics and diarrhea. Ann Nutr Metab, ۲۰۱۰. ۵۷(Suppl):۱۰–۱۱ ...
Chen, X., X.M. Liu, F. Tian, et al. Antagonistic activities ...
Rijkers, G.T., S. Bengmark, P. Enck, et al. Guidance for ...
Rizzello, C.G., A. Cassone, R. Coda, et al. Antifungal activity ...
Delavenne E, Mounier J, Déniel F, Barbier G, Le Blay ...
Wen K, Azevedo MS, Gonzalez A, et al. Toll-like receptors ...
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