Effects of Combination of G-CSF and SCF One Week Prior to Liver Injury In Acute liver Damage Model Induced by Thioacetamide Administration

Background: There are many reports regarding to effects of Granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF)alone in liver repair .But conflicting data have been reported regarding the role of growth factors such as G-CSF and SCF in the liver regeneration system. Also, there is not such data regarding to effects of co-administration both of G-CSF and SCF in the liver damage condition. Materials and Methods: An experimental model of rat liver damage induced by the thioacetamide. Five different groups of animals receiving ۰.۹% NaCl, TAA alone, TAA + G-CSF, TAA + SCF and TAA + (G-CSF+SCF ). The activity of glutamate pyruvate transaminase (GPT/AlT)and glutamate oxaloacetate transaminase (GOT/AST) were measured after the thioacetamide (TAA) injection and the administration of combination of G-CSF + SCF for ۱۲ weeks. Also histological tests were carried out at the end experiments. Results: The pre-treatment of combination of G-CSF and SCF for ۱۲ weeks reduced the degree of liver injury. The mean of GOT activity was ۶۱.۲۴ (U/L) in the G-CSF +SCF and versus ۱۳۲.۸۶ in the TAA-alone group. These differences in the GOT activity were statistically significant (P<۰.۰۵). Also, in the G-CSF +SCF and TAA group the mean of GPT activity (۴.۳۵ versus ۱۱.۷۹, respectively) were lower than in the TAA-alone group, this difference was statistically significant (P<۰.۰۵). Liver sections from a rat treated only with TAA, showing damage, but TAA and G-CSF + SCF no significant damage is present. On the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of G-SCF+SCF and TAA-treated rats compared to TAA only treated group. Conclusion: Biochemical and microscopic analysis revealed that combination of G-CSF and SCF pre-treatment significantly enhances liver regeneration after TAA –induced liver injury.

Keywords:

Damage , Granulocyte colony-stimulating factor , stem cell factor , thioacetamide , glutamate pyruvate transaminase , glutamate oxaloacetate transaminase