Association between the Asp۳۲۷Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer

Background: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. Since the genetic changes in the structure of globulin affect it’s binding to hormones, so in this study the effects of single nucleotide change in exon ۸ or rs ۶۲۵۹ in the incidence of prostate cancer is evaluated. Methods: The study population included ۱۲۰ patients with prostate cancer and ۱۲۰ control subjects. After collecting blood samples, DNA was extracted by salting out the method in order to determine the genotype of individuals by RFLP-PCR method. According to Hardy-Weinberg equilibrium genotypes and allele frequencies were calculated and a relationship between this variation and prostate cancer were evaluated and by using SPSS ۲۳ software and the relationship between variations. The significance level was considered ≤۰.۰۵. Results: Results indicated that homozygous mutant genotype AA ۲.۵۸ (p value= ۰.۰۰۷, OR: ۲.۵۸, CI۹۵%: ۱.۵۲-۴.۳۸) and heterozygous AG ۱.۱۸ times (p-value =۰.۵, OR: ۱.۱۸, CI۹۵%: ۰.۳۸-۳.۶۱) increase chance of getting prostate cancer in carriers. But homozygous of wild genotype GG have the protective role against prostate cancer (p-value =۰.۰۰۵, OR: ۰.۳۸۵, CI۹۵%: ۰.۲۳-۰.۶۵). Conclusion: Therefore, Asn allele is one of the main factors in prostate cancer so it can be used as the non-invasive and suitable marker for early detection in susceptible individuals.

Keywords:

Prostate cancer , Sex hormone-binding globulin , PCR-RFLP