Evaluation of Long Stress-Induced Non-coding Transcripts ۵ Polymorphism in Iranian Patients with Bladder Cancer

Background: Bladder cancer (BC) is the most commonly diagnosed genitourinary cancer in Iran, presented in both men and women. BC is a multifactorial trait resulting from the complex interaction between several genes and environmental factors. Long stress-induced non-coding transcript ۵ (LSINCT۵), a member of the long non-coding RNAs, is abundantly expressed in high proliferative cells, as well as the cells vulnerable to cellular stress in response to chemical carcinogens. This case-control study aimed to determine any association between LSINCT۵ rs۲۹۶۲۵۸۶ polymorphism and bladder cancer. Materials and Methods: A group of ۱۵۰ patients with BC were compared with ۱۴۳ subjects as a control group. Genotyping of the rs۲۹۶۲۵۸۶ polymorphism was done using tetra- primer amplification refractory mutation system-polymerase chain reaction (T-ARMS PCR) method. Results: Genotype and allele distribution were not significantly different between the case and control groups. Smoking was found to be the confounding risk factor for bladder cancer. Conclusion: Considering the result of our analyses, it seems that LSINCT۵ could not affect individual susceptibility to BC among Iranian patients, however, it can be considered as a disease predictor among smokers.

Keywords:

Bladder cancer , Long stress induced non-coding transcript ۵ , Polymorphism