An Efficient Protocol for Embryonic Carcinoma Cells P۱۹ Differentiation to Cardiomyocytes Using Oxytocin as Inducer

Background: The capability of embryonic carcinoma cells P۱۹ in differentiation to Cardiomyocyte was examined through inducing effects of Oxytocin (OT) and ۵-Azacytidin (۵Az) individually and compared with each other in laboratory condition. Materials and Methods: P۱۹ Embryoid Bodies (EBs) was formed through hanging drops method. Then, EBs were treated with (۵Az) or (OT) and the EB medium (Ctrl) until ۱۲ days. Morphology and beating number per minute were recorded every two days. Viability was carried out every three days. The expression of several cardiomyocyte-associated genes was assessed by RT-PCR. Results: The beating area percentage of EBs in OT treatment groups was more than that of the ۵Az group in all days of experiment. However, only in final stage, a significant increase was observed in beating area of OT group. There was no significant difference in viability and morphological changes. OT induction expressed three more specific proteins in cell culture than ۵Az. Conclusion: Statistical analysis revealed that response to OT inducer was more excessive than ۵Az in all treatment groups. The Oxytocin was found to be effective inducer of cardiomyocytes differentiation from embryonic carcinoma cells P۱۹ than ۵-azacytidine.

Keywords:

P۱۹ Cells , Embryoid Bodies , Cardiomyocytes , ۵-Azacytidin , Oxytocin , Differentiation