Study of Cytochrome P۴۵۰ ۱A۱ (T۳۸۰۱C) Single Nucleotide Polymorphism in Patients with Breast Cancer in Mazandaran Province-Northern Iran

Background: Breast cancer is the first leading cause of cancer-related death in women. Pesticides which are excessively used in northern Iran are one of the most important risk factors for breast cancer incidence. The cytochrome P۴۵۰ ۱A۱ (cyp۱A۱) is a key enzyme in xenobiotics metabolism and SNPs of its coding gene has been verified to be important in cancer susceptibility. The aim of this study was to evaluate the association of cyp۱A۱ M۱ polymorphism with the risk of breast cancer in Mazandaran province. Materials and Methods: Ninety six breast cancer patients with known clinopathological characters and ۱۱۰ healthy women as control were genotyped for cyp۱A۱ M۱ polymorphisms by PCR-RFLP technique using Msp۱ restriction enzymes. Logistic regression model was applied for statistical analysis. Results: The frequency of TT and TC genotypes of M۱ polymorphism was calculated ۸۶, ۱۴% for cases and ۷۹ and ۲۱% for control group, respectively. Surprisingly, the mutant CC genotype was not found in any subjects. Statistical analysis showed no significant correlation between allelic variants and breast cancer risk (p value= ۰.۴۲, OR=۰.۶۶, CI= ۰.۲۴-۱.۸۱). No significant correlation was also found between genotypic frequency and clinopathological characters. Conclusion: Only TT and TC genotypes were found in the studied subjects. The M۱ allelic variants were significantly associated neither with breast cancer risk nor with clinopathological characteristics.

Keywords:

Breast cancer , cyp۱A۱ gene , Single nucleotide polymorphism , Pesticide