The Effect of Aerobic Training with Vitamin D on Extrinsic Pathway of Apoptosis and Anti- Apoptotic Indices of Heart Tissue of Rats Exposed to Oxidative Damage Induced by H۲O۲

Introduction: Apoptosis plays an important role in the development of cardiovascular diseases. The aim of this study was to investigate the effect of aerobic training (AT) with Vitamin D (VD) on extrinsic pathway of apoptosis and anti- apoptotic indices of heart tissue of rats exposed to oxidative damage induced by H۲O۲.Methods: In this experimental study, ۶۰ rats were selected and randomly placed into ۱۰ groups of six, including ۱) ۱ mmol / kg H۲O۲, ۲) ۲ mmol / kg H۲O۲, ۳) VD + ۱ mmol / kg H۲O۲, ۴) AT + ۱ mmol / kg H۲O۲, ۵) AT + VD + ۱ mmol / kg H۲O۲, ۶) VD + ۲ mmol / kg H۲O۲, ۷) AT + ۲ mmol / kg H۲O۲, ۸) AT + VD + ۲ mmol / kg H۲O۲, ۹) sham and ۱۰) control. During eight weeks, the rats in groups ۱-۹ were subjected to intraperitoneal injection of H۲O۲ at specific doses three times a week; the rats in groups ۴, ۵, ۷ and ۸ performed aerobic trainings, and the rats in groups ۳, ۵, ۶ and ۸ received ۰.۵ μg / kg of vitamin D۳ intraperitoneally daily. Protein concentrations of Caspase ۸, Fas and FLIP were measured in rat heart tissue. For analysis of the research findings, Kolmogorov- Smirnov, one-way ANOVA, two-way ANOVA along with Bonferroni post hoc tests were used (p≤۰.۰۵). Results: H۲O۲ has a significant effect on the increase of Caspase ۸, Fas and FLIP in the heart tissue of rats (p≤۰.۰۵); eight weeks of aerobic training and Vitamin D intake has a significant effect on decreasing the concentration of Caspase ۸, Fas and increase of FLIP in the heart tissue of rats exposed to oxidative damage induced by ۱ and ۲ m/mol/kg H۲O۲ (p ≤۰.۰۵); aerobic training and Vitamin D intake has significant interactions in the reduction of Caspase ۸, Fas in the heart tissue of rats exposed to oxidative damage induced by ۱ and ۲ m/mol/kg H۲O۲ (p≤۰.۰۵), however, it does not have interactive effect on the increased FLIP of the heart tissue of rats exposed to oxidative damage induced by ۱ and ۲ m/mol/kg H۲O۲ (p≥۰.۰۵). Conclusion: It seems that performing aerobic training activities and simultaneous consumption of vitamin D supplement has interactive effects on reducing some of the extrinsic pathway indices of apoptosis in the heart tissue of H۲O۲-rats exposed to oxidative damage induced by H۲O۲.

Keywords:

Training , Vitamin D , Apoptosis , H۲O۲