Hyperglycemia Induction in HepG۲ Cell Line

Background: Hyperglycemia is one of the important featurs of diabetes. In cell culture studies different methods are used to mimic the hyperglycemia condition. In this study we investigate response of human liver cancer cell line (HepG۲) to high insulin, high glucose, and high insulin/ high glucose medium exposure.Methods: HepG۲ cells were settled in DMEM+۰.۱% FBS or DMEM free-serum medium with high concentrations of d-glucose (۳۰ mm) and/or insulin (۱μM) for ۲۴h after an overnight starving in serum- free medium. The level of hyperglycemia was estimated in the supernatants via GOD-POD method.Results: Serum-free madium with high insulin/ high glucose consentration made the higher level of hypreglycemia in HepG۲ cells.Conclusions: Our study interduced high insulin/ high glucose treatment as the best way to induction hyperglycemia.Background: Hyperglycemia is one of the important featurs of diabetes. In cell culture studies different methods are used to mimic the hyperglycemia condition. In this study we investigate response of human liver cancer cell line (HepG۲) to high insulin, high glucose, and high insulin/ high glucose medium exposure. Methods: HepG۲ cells were settled in DMEM+۰.۱% FBS or DMEM free-serum medium with high concentrations of d-glucose (۳۰ mm) and/or insulin (۱μM) for ۲۴h after an overnight starving in serum- free medium. The level of hyperglycemia was estimated in the supernatants via GOD-POD method. Results: Serum-free madium with high insulin/ high glucose consentration made the higher level of hypreglycemia in HepG۲ cells. Conclusions: Our study interduced high insulin/ high glucose treatment as the best way to induction hyperglycemia.

Keywords:

Hyperglycemia , HepG۲ , Insulin , Glucose , diabetes.