The Relationship between Torque teno Virus and TLR۲ rs۵۷۴۳۷۰۸ Polymorphism with Breast Cancer

Introduction: Breast cancer is one of the most important causes of mortality in women. Various factors are involved in the development of cancer, including viruses. Toll-like receptors (TLRs) have an essential role in the innate immune system. The present study investigated the relationship between TLR۲ rs۵۷۴۳۷۰۸ polymorphisms and Torque teno virus (TTV) infection with breast cancer. Methods: Blood samples from ۸۰ women with breast cancer and ۸۰ healthy women were collected, and after DNA extraction, the presence of TTV was investigated by a PCR assay and polymorphism in the TLR۲ gene (rs۵۷۴۳۷۰۸) was explored using the PCR-RFLP method. Also, the physical and chemical properties of TLR۲ protein in the two wild and mutant forms were analyzed using the ExPASy database. Results: Statistical analysis showed that there was no significant relationship between the age and TTV infection; TTV infection and breast cancer; the grade of cancer, and TTV infection; while there were significant relationships between rs۵۷۴۳۷۰۸ polymorphisms and breast cancer; GG genotype and increased incidence of cancer; TTV infection and rs۵۷۴۳۷۰۸ polymorphisms. Also, instability index, aliphatic index, grand average of hydropathicity, and molecular weight of TLR۲ protein varied in wild and mutant states. Conclusions: Although there was no significant relationship between TTV infection and breast cancer, the rs۵۷۴۳۷۰۸ polymorphisms might be involved in TTV infection and breast cancer.

Keywords:

Virus , Single nucleotide polymorphisms , breast cancer , Toll-like receptor