Detection of DNA Gyrase Mutation and Multidrug Efflux Pumps Hyperactivity in Ciprofloxacin Resistant Clinical Isolates of Pseudomonas aeruginosa

Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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JR_JMMI-1-1_001

تاریخ نمایه سازی: 29 بهمن 1402

Abstract:

Target modification and reduced drug accumulation are the main resistance mechanisms against fluoroquinolone antibiotics in Pseudomonas aeruginosa. We performed a genotypic characterization of three major Mex multidrug efflux pumps (MexAB-OprM, MexXY-OprM and MexCD-OprJ) in ciprofloxacin resistant clinical isolates of P. aeru­ginosa, collected from Tehran, Iran this was followed by sequencing and analyzing the type II topoisomerases en­coding gyrA, gyrB, parC , and parE genes. Reverse transcription PCR (RT-PCR) and semi-quantitative RT-PCR methods were used to analyse the transcription of efflux pumps. Topoisomerase mutation analysis was carried out through PCR amplification and sequencing of the quinolone resistance determining region (QRDR) of the topoiso­merases encoding genes. Some ۱۱.۱% of the strains actively expressed MexCD-OprJ and ۱۵.۵% hyperexpressed MexXY-OprM efflux pumps. No overexpression was detected for MexAB-OprM, whereas ۴.۴% of strains showed simultaneous expression of MexCD-OprJ and MexXY-OprM. In the sequencing results, a single point mutation in the QRDR of gyrA was detected in all tested strains, where Isoleucine was substituted by Threonine at position ۸۳. No mutations were detected in QRDR of gyrB, parC and parE genes. We are the first to report the genotypic analysis of ciprofloxacin mediated efflux pump resistance from Iran. These findings emphasize the clinical significance of multidrug efflux pumps and topoisomerases mutations activity in conferring resistance to fluoroquinolone antibiotics, and support the use of genotypic methods for analysis of resistance elements in P. aeruginosa in developing countries such as Iran.

Authors

Abolghasem Tohidpour

Department of Medical Bacteriology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Shahin Najar Peerayeh

Department of Medical Bacteriology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Sarah Najafi

Pharmaceutical Incubator Centers, Tehran University of Medical Sciences, Tehran, Iran

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  • Rossolini GM, Mantengoli E. Treatment and control of severe infections ...
  • Bonomo RA, Szabo D. Mechanisms of multidrug re-sistance in acinetobacter ...
  • Nikaido H. Multidrug resistance in bacteria. Annu Rev Biochem. ۲۰۰۹; ...
  • Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical ...
  • Schweizer HP. Efflux as a mechanism of resistance to antimicrobials ...
  • Davis R, Markham A, Balfour JA. Ciprofloxacin. An updated review ...
  • Grangie JD, Roulot D, Pelletier G, Pariente EA, Denis J, ...
  • Giamarellou H, Antoniadou A. Antipseudomonal antibiotics. Med Clin North Am. ...
  • Jones RN, Beach ML, Pfaller MA. Spectrum and activity of ...
  • Yoshida H, Bogaki M, Nakamura M, Nakamura S. Quinolone resistance-determining ...
  • Cambau E, Perani E, Dib C, Petinon C, Trias J, ...
  • Ruiz J. Mechanisms of resistance to quinolones: target alterations, decreased ...
  • Mesaros N, Nordmann P, Plesiat P, Roussel-Delvallez M, Van Eldere ...
  • Poole K. Efflux-mediated resistance to fluoroquin-olones in gram-negative bacteria. Antimicrob ...
  • Li XZ, Barre N, Poole K. Influence of the MexA-MexB-OprM ...
  • Van Bambeke F, Glupczynski Y, Plesiat P, Pechère JC, Tulkens ...
  • Piddock LJ. Multidrug-resistance efflux pumps? not just for resistance. Nat ...
  • Poole K, Gotoh N, Tsujimoto H, Zhao Q, Wada A, ...
  • Piddock LJ. Clinically relevant chromosomally en-coded multidrug resistance efflux pumps ...
  • Tohidpour A, Najar Peerayeh S, Mehrabadi JF, Rezaei Yazdi H. ...
  • Dunham SA, McPherson CJ, Miller AA. The relative contribution of ...
  • Llanes C, Hocquet D, Vogne C, Benali-Baitich D, Neuwirth C, ...
  • Dumas JL, Van Delden C, Perron K, Kohler T. Analysis ...
  • Linares JF, López JA, Camafeita E, Albar JP, Rojo F, ...
  • Llanes C, Köhler T, Patry I, Dehecq B, Van Delden ...
  • Mesaros N, Glupczynski Y, Avrain L, Caceres NE, Tulkens PM, ...
  • Lee A, Mao W, Warren MS, Mistry A, Hoshino K, ...
  • Pasca MR, Dalla Valle C, De Jesus Lopes Ribeiro AL, ...
  • Jalal S , Ciofu O, Høiby N, Gotoh N, Wretlind ...
  • Akasaka T, Tanaka M, Yamaguchi A, Sato K. Type II ...
  • Poole K, Srikumar R. Multidrug efflux in Pseudomonas aeruginosa: components, ...
  • Akasaka T, Onodera Y, Tanaka M, Sato K. Cloning, expression ...
  • Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J, ...
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