Detection of Anti-IgGs against Heat Shock Proteins ۲۷ and ۲۰, HP۹۱ Peptide, and HIV-۱ Polypeptides in HIV-Positive and Negative Patients

Introduction: A simple and sensitive diagnosis method is needed to identify HIV infection in sera of untreated, treated, and drug-resistant patients. The purpose of this study is to determine whether heat shock proteins (Hsp)-۲۷ and -۲۰ and HP۹۱ peptide along with HIV-۱ polypeptides can serve as potential biomarkers to distinguish HIV infection in untreated, treated, and drug-resistant individuals compared to HIV-negative subjects. Methods: At first, human sera were obtained from ۱۴۱ participants, including ۲۰ naïve HIV-infected, ۷۱ treated, ۳۰ drug-resistant, ۲۰ HIV-negative (healthy/control) individuals. The recombinant Hsp۲۷, Hsp۲۰, and five designed HIV-۱ polypeptides were expressed in Escherichia coli and purified by affinity chromatography under denaturing or native conditions. Finally, the antibodies against these antigens were quantified in sera using ELISA. Results: Our data showed that HIV-infected patients significantly displayed higher serum levels of anti-Hsp۲۷, anti-HP۹۱, and anti-Nef-Tat-Gp۱۶۰-P۲۴, anti-Nef-Vpr-Gp۱۶۰-P۲۴, anti-Nef-Vif-Gp۱۶۰-P۲۴, anti-Nef-Vpu-Gp۱۶۰-P۲۴, and anti-Nef-Rev-Gp۱۶۰-P۲۴ polypeptide antibodies than healthy groups (p < ۰.۰۵), but not for anti-Hsp۲۰. Moreover, the serum levels of antibodies against Hsp۲۷, Hsp۲۰, HP۹۱, and HIV-۱ polypeptides were not statistically significant between different groups of patients (p > ۰.۰۵). Conclusion: The levels of anti-Hsp۲۷ and anti-HP۹۱ antibodies in serum increased in HIV-۱ seropositive subjects along with antibodies against five HIV-۱ polypeptides suggesting their potential value as a diagnostic marker for HIV-۱ infections.

Keywords:

HIV-۱ proteins , Heat shock proteins , HP۹۱ peptide , Antibody detection