Molecular Study of Occult Hepatitis C Infection among Iranian Hemophilia Patients Treated with Direct-acting Antiviral Agents

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JMMI-8-1_001

تاریخ نمایه سازی: 29 بهمن 1402

Abstract:

Introduction: A new pathological form of HCV named as occult HCV infection (OCI) has been recently characterized by the presence of HCV RNA in liver biopsy and/or peripheral blood mononuclear cell specimens (PBMCs) and the absence of detectable circulating HCV RNA in plasma samples. In this study, we investigated the presence of HCV RNA in PBMCs and plasma samples of ۱۰۰ hemophilia patients with negative serum HCV RNA. Methods: One hundred hemophilia participants receiving IFN-free direct-acting antivirals (DAAs) regimens as a treatment of HCV infection participated in this study. PBMCs were separated with Ficoll before RNA extraction. The HCV genotypes of the positive specimens were also analyzed by RT-PCR assay. Finally, data analysis was performed by SPSS software. Results: Our data revealed that out of ۱۰۰ hemophilia patients, three (۳%, ۹۵% CI: ۰.۰۰۶-۰.۰۸۵) were positive for OCI, showing a significant association between OCI and genotype۳/drug regimens (p = ۰.۰۲۰۳). There was no significant increase at ALT and AST levels in patients with OCI. Moreover, a genotype difference was observed between plasma and PBMCs samples of ۱% (۱/۱۰۰) of patients. Conclusion: Generally, HCV genotyping in PBMCs along with plasma subtyping before beginning the therapy is vital due to the possibility of OCI detection.

Authors

Elnaz Agi

Iranian Comprehensive Hemophilia Care Center, Tehran, Iran

Saber Asghari

Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Ali Namvar

Iranian Comprehensive Hemophilia Care Center, Tehran, Iran

Niloofar Khairkhah

Iranian Comprehensive Hemophilia Care Center, Tehran, Iran

Niloofar Naderi

Iranian Comprehensive Hemophilia Care Center, Tehran, Iran

Ali Anvar

Iranian Comprehensive Hemophilia Care Center, Tehran, Iran

Alireza Azizi Saraji

Iranian Comprehensive Hemophilia Care Center, Tehran, Iran

Azam Bolhassani

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran

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