Correlation of Biofilm Formation and Caco-۲ Cell Attachment Properties in Colonization Ability of Acid-Bile Resistant Fecal Lactobacillus plantarum Isolates
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JMMI-6-1_003
تاریخ نمایه سازی: 29 بهمن 1402
Abstract:
Introduction: The strain-specific capabilities of lactobacilli are critical for gut colonization. In this study, we evaluated various colonization determinants of ۴۲ fecal Lactobacillus plantarum isolates from the healthy human fecal samples. Methods: We investigated the attachment to the Caco-۲ cell line, biofilm formation ability and cell surface activity of the isolates. Such properties were comparatively studied, and the regression between these features was statistically analyzed. Results: Among our results ۱۸ (۴۲.۹%) were non-adhesive, ۱۱ (۲۶.۲%) moderate adhesive and ۱۳ (۳۱%) strongly adhesive. The results showed that some isolates were significantly capable of biofilm formation. The highest rate of auto-aggregation was recorded for L. plantarum isolate RPR ۲۴۰ (۵۸%). Conclusion: Our results revealed a high degree of variability among colonization characteristics of the L. plantarum isolates indicating strain-specificity. Also, the colonization properties of the potential probiotic isolates had no significant correlation with each other. Our results confirmed the necessity of further in vivo colonization assays for selection of probiotic candidates.
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Authors
Mahdi Rohani
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Moslem Papizadeh
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Mohammad Reza Pourshafie
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
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