Study of the antibacterial efficacy of tetracycline-conjugated gold nanoparticles

BACKGROUND AND OBJECTIVESThe emergence of antibiotic-resistant bacteria has become a growing concern in healthcare and necessitates the development of novel strategies to combat these pathogens. In this study, we investigate the antibacterial activity of tetracycline conjugated gold nanoparticles (Tc-Au NPs). Gold nanoparticles have gained interest due to their unique physicochemical properties, including their ability to efficiently deliver drug payloads.MATERIALS AND METHODSIn this study, we conjugate tetracycline, a broad-spectrum antibiotic, onto the surface of gold nanoparticles to improve its efficacy against bacterial pathogens. Formation of nanoparticles was determined using visible-UV spectrophotometer, Fourier-transform infrared spectroscopy, and transmission electron microscopy. The Mueller-Hinton broth environment was used for minimum inhibitory concentration (MIC) inhibition of Tc-Au NPs.RESULTS AND DISCUSSIONOur results demonstrate that Tc-Au NPs display enhanced antibacterial activity compared to free tetracycline, with lower minimum inhibitory concentrations observed. Additionally, the cytotoxicity of Tc-Au NPs is evaluated using in vitro cell viability assays. The results reveal that Tc-Au NPs exhibit low cytotoxicity, indicating their potential as a safe and effective antibacterial therapy.CONCLUSIONOur findings provide valuable insights into the potential use of tetracycline conjugated gold nanoparticles as an alternative antibacterial therapy with improved efficacy and safety profiles. Overall, this study contributes to the ongoing efforts in combating antibiotic resistance and offers a promising platform for the development of nanoparticle-based antibacterial agents.

Keywords:

Tetracycline antibiotics , gold nanoparticles , synthetic approach , antibacterial activity.