Histopathological Assessment of Testicular Tissues from Mice with Xenografted Human Triple-Negative Breast Cancer

Introduction: Mammary tumors are a common cancer in human but little is known about their histopathological characteristics and effects on other organs. Understanding tumor effects on testicular tissue has implications for developing mouse models of human breast cancer.Methods: Nude mice were divided into two groups, with ۵ mice implanted with mammary tumor cells (experimental group) and ۵ mice serving as controls. After tumor growth, histopathological analysis of the tumors and testes was performed using hematoxylin and eosin staining. Several features were qualitatively scored in tumor and testis sections including mitosis, necrosis, angiogenesis, inflammation, hyperemia, and atrophy.Results: Tumors from experimental mice showed significantly higher mitotic rate, necrosis, angiogenesis, inflammation, and atrophy compared to controls. Multiple differences were observed in testis pathology compared to a control group. Experimental tumors displayed more aggressive qualities.Conclusion: This study demonstrates that mammary tumors generated in nude mice exhibit elevated pathological characteristics tied to aggressiveness, including proliferation and cell death, compared to control mice. Notably, tumor growth affected testis histology including atrophy and hyperemia. These results provide insights into using this mouse model of breast cancer and establish a baseline for pathological features of experimental mammary tumors versus healthy tissues.

Keywords:

breast cancer , MDA-MB-۲۳۱ xenograft model , nude mice