Study of salmonella infection in broiler turkey carcasses in some provinces in Iran; molecular detection and antimicrobial susceptibility pattern

Salmonella, as one of the most important zoonotic foodborne pathogens, is a major global threat to the health of human communities and animals and its impact is economically significant in the world. This study aimed to investigate Salmonella infection and bacterial susceptibility test on possible isolates in some broiler turkey flocks in Iran. For this purpose, diagnostic samples were collected at slaughterhouse from ۱۵ different broiler turkey flocks at the ages of ۱۶ to ۱۸ weeks, from different geographical regions of the country. In this regard, ۱۵ complete intestinal samples were taken from each flock and a total of ۲۲۵ intestinal samples were collected and sent to the laboratory for culture, isolation and identification of Salmonella. From every ۵ intestinal samples different parts were pooled and cultured in selenite F medium and transferred to Salmonella Shigella agar (SS) and MacConkey agar (MAC) media after ۲۴ hours, then suspected Salmonella colonies were transferred to the differential medias. Salmonella positive colonies were used for serogroup identification and all isolates were identified being from D serogroup.The PCR were performed for identification of Salmonella spp and ۷ Salmonella isolates were confirmed. Then the PCR with specific primers for the identification of Salmonella Enteritidis was performed on ۵ isolates and ۴ isolates were identified as Salmonella Enteritidis.In addition, antimicrobial susceptibility test was performed on ۷ isolates and it was found that all of them were completely sensitive to Fosfomycin and the highest resistance was observed in Colistin.

Keywords:

foodborne pathogens , slaughterhouse , culture , PCR , Salmonella Enteritidis