The ability of uropathogenic Escherichia coli isolates in biofilm formation on urinary catheter and association with bcsA and draE genes

The product biofilm by E.coli strain can cause serious problem for human health. Catheter is considered to be s suitable place for colonization of microorganisms, so that in %۵-۱۰ of patients who used catheter for only one day and also in all patients who used it for over ۲۸ days, this colonization of bacteria is observed on catheter. This study is come out in order to compare biofilm formation in E.coli and its relationship with pathogenic factors. In this study ۱۴۴ isolates of uropathogenic E.coli were used in order to compare their potential of biofilm formation on urinary catheter. To do this, a piece of the catheter was sliced and put in tubes containing E.coli suspension, as well as in broth medium (as witness). After incubation at ۳۷ oC for ۲۴ hours, the number of live bacteria involve in biofilm formation was counted by surface cultivation method and then studied. The result showed that among the ۱۴۴ studied E.coli isolates, ۱۳۰ (%۸۹.۷) had cellulose synthesized gene (bcsA), and ۲۲(%۱۵.۲) had Dr adhesine gene (draE). There was a significant relationship between bcsA gene and biofilm formation potential on urinary catheter.

Keywords:

E.coli (UPEC) , Biofilm , cellulose synthase , Dr adhesine , catheters