A comparison of culture and PCR methods for identifying Moraxella catarrhalis in patients with respiratory diseases

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJMCM-8-1_005

تاریخ نمایه سازی: 19 اسفند 1402

Abstract:

Moraxella catarrhalis is considered as one of the most significant pathogens of the respiratory tract. This research aimed to isolate and identify M. catarrhalis in respiratory diseases patients from northern part of Iran using culture and PCR techniques. In this study, ۲۸۰ samples including throat swab of the patients with pharyngitis (n=۹۲), sinus secretions of the patients with sinusitis (N=۸۵), ear secretions of patients with otitis media (n=۴۳) and pulmonary secretions of the patients hospitalized in the intensive care unit (n=۶۰) were collected. Culture technique and phenotyping tests were used to isolate and identify the bacterium. PCR technique was also used for its identification by usage of the specific primers. Of a total of ۲۸۰ samples, ۲۷ samples (۹.۶۴%), and ۸۷ samples (۳۱.۰۷%), were reported to be positive in terms of presence of M. catarrhalis using culture and PCR techniques, respectively. The results obtained by this study show that M. catarrhalis is present in the human societies and hospital environments. Therefore, rapid identification and tracking of its strains can play a significant role in prevention from their development.

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Authors

Mina Eghbali

Department of Microbiology, Fars Science and Research branch, Islamic Azad University, Fars, Iran. Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

majid Baserisalehi

Department of microbiology, Kazeron branch, islamic azad university, Kazeron, Iran.

masood Ghane

Department of Microbiology, Tonekabon Branch, Islamic Azad university, tonekabon, Iran.