Investigating polymorphisms of SAR1B and BMP4 genes and their effect on fat and tail in Qazel sheep.

In the present research, to identify the polymorphism of exon two of BMP4 gene and exon 5 of SAR1B gene in the Qazel sheep population, blood samples were taken from 135 Qazel sheep. An optimized salt method was used for DNA extraction. 1% agarose gel was used to determine the quality of DNA. Exon number two of BMP4 gene and exon 5 of SAR1B gene were amplified by a pair of specific primers. Amplification of 360 bp fragment of exon 2 of BMP4 gene and 481 bp fragment of exon 5 of SAR1B gene was performed using polymerase chain reaction. In order to identify polymorphism (polymorphism) and determine genetic patterns, the method based on the SSCP marker (Different Spatial Form of Single Strands) was used. SSCP genetic patterns for BMP4 gene were determined using vertical electrophoresis on 12% acrylamide gel for 6.30 hours at 300 V and for SAR1B gene for 16 hours at 220 V at 4°C. Silver nitrate was used to stain the acrylamide gel. Examining the gene patterns of exon 2 of the BMP4 gene indicated the presence of polymorphisms in the gazelle breed, which showed 4 different patterns. Also, patterns related to exon 5 of SAR1B gene in deer breed were monomorphic and had no effect on tail fat.

Investigating polymorphisms of SAR1B and BMP4 genes and their effect on fat and tail in Qazel sheep. Keywords:

polymorphism , BMP4 and SAR1B gene , PCR-SSCP , sheep's fat and tail