The Effect of Exposure to Hypoxia during Resistance Training On the Concentration of PAX۷ and PGC-۱α Proteins in Fast-Twitch Muscle Tissue of Diabetic Rats Fed a High-Fat Diet

Introduction: Myopathy caused by diabetes can accelerate the disease process in diabetic people. Myopathy has important indicators in muscle tissue that are related to regeneration and intracellular metabolism in skeletal muscles. The aim of current study was to investigate the effect of ۸ weeks of resistance training in hypoxia on the content of PAX ۷ and PGC-۱α proteins in the gastrocnemius muscle of type ۲ diabetic rats. Methods: The present study was of an experimental type in which ۲۴ male Wistar rats ۶ weeks after induction of type ۲ diabetes were divided into three groups: healthy control (HC), diabetic control (DC), and hypoxia group (HPX). Resistance training was applied for ۸ weeks under oxygen deficiency conditions in the groups of resistance training in hypoxia. After finishing the exercises, the tissue sample was taken from the biceps muscle and evaluated to measure the concentration of PAX ۷ and PGC-۱α proteins, and the results were analyzed by one-way analysis of variance at a significance level ≥۰.۰۵α. Results: The results showed that there was a significant difference (P=۰.۰۰۰۱) between the research groups in both PAX۷ and PGC-۱α proteins. Induction of diabetes led to a significant decrease in PAX۷ compared to the control group. PGC۱-α protein levels also decreased significantly in the diabetes induction group compared to the control group (P=۰.۰۰۰۱), but exposure to hypoxia did not change the gene expression values of this variable compared to the diabetic patient group (P = ۰.۴۵۱). Conclusion: Exposure to temporary and passive hypoxia can be considered as a suggested strategy to improve indicators related to type ۲ diabetes in humans.

Keywords:

Diabetic myopathy , Hypoxia , PAX۷ , PGC-۱α