The Effects of R۱۰ Fraction of Garlic (Allium sativum L.) on Gonadotropins and Oocyte Maturation in a PCOS Mouse Model

Introduction: Polycystic ovary syndrome (PCOS) is a common metabolic, immunological, and reproductive disorder worldwide. Successful therapeutic and management approaches for PCOS remain a challenge. This experimental study was designed to find out the effects of garlic R۱۰ fraction on gonadotropins, oocyte-secreted factors, and oocyte maturation in a PCOS Mouse Model.Materials and Methods: R۱۰ fraction was obtained from garlic extract and PCOS-induced mice were prepared using estradiol valerate. Treatment with R۱۰ fraction was performed and compared between two the normal and control PCOS groups. The alterations in gonadotropins (LH and FSH), oocyte-secreted factors gene expression (GDF۹ and BMP۱۵), and evaluation of histological changes in the ovary were performed.Results: After treatment with R۱۰ fraction, the LH level was increased and conversely the FSH level was decreased in the serum of the PCOS model. Furthermore, PCOS-induced ovaries demonstrated high atrophy and a significant decrease in the number of Graaf. There was also a significant reduction in the GDF۹ and BMP۱۵ gene expression levels in the PCOS model compared to the normal group (p <۰.۰۰۱).Conclusions: This study indicates that garlic R۱۰ fraction could modulate PCOS in mice at the systemic level in the hypothalamic-pituitary-gonadal axis and the local level in the ovaries. Accordingly, the R۱۰ fraction can be considered a therapeutic approach to improve PCOS conditions.

Keywords:

Polycystic ovary syndrome , Garlic R۱۰ Fraction , gonadotropin , Gene Expression , Mouse Model