Effect of sodium nitroprusside pretreatment on the improvement of physiological and biochemical characteristics of soybean (Glycine max L.) Merrill) (cv. Williams) seeds under the influence of accelerated aging

In order to investigate the effect of sodium nitroprusside pretreatment on the improvement of physiological and biochemical characteristics of soybean seed cv. Williams under the influence of accelerated aging, a factorial experiment was conducted based on a completely randomized design with three replications at the University of Mohaghegh Ardabili in ۲۰۲۳. Experimental treatments included accelerated aging treatment at three levels (control, ۲۴ and ۴۸ hours) and sodium nitroprusside at three levels (control, ۱۰۰ and ۲۰۰ ppm). The results showed that aging reduced growth indices including Germination Percentage (GP), Allometric Coefficient (AC), Radicle length (RL), Plumule length (PL) and seedling length (SL), Radicle and Plumule Fresh Weight (RFW and PFW), Radicle and Plumule Dry Weight (RDW and PDW), Seed Weight Vigor Index (SWVI) and Seed Length Vigor Index (SLVI), but seed pretreatment with different levels of sodium Nitroprusside, especially the level of ۲۰۰ ppm, improved these traits. Pretreatment with sodium nitroprusside decreased the amount of malondialdehyde, so the highest content of malondialdehyde (۱۰.۳۶ μmol/g) observed in the control treatment (priming with distilled water). Glutathione reductase enzyme activity increased by ۲۵% in pretreatment with sodium nitroprusside ۲۰۰. The activity of the lipase enzyme and protein content with pretreatment sodium nitroprusside ۲۰۰ ppm and without aging was increased by about ۶۳ and ۶۶%, respectively. The results showed that seed treatment with sodium nitroprusside ۲۰۰ ppm is the most effective method to improve soybean seed growth indicators, and by stimulating antioxidant enzymes and neutralizing free radicals, it can reduce the harmful effects of aging on some traits in soybean seedlings and improve seedling growth.

Keywords:

Aging , Biochemical traits , Germination Indicators , Priming , Sodium Nitroprusside , پرایمینگ , سدیم نیتروپروساید , شاخص های رشد , صفات بیوشیمیایی و فرسودگی