Effects of Curcumin Nanoparticles on the Tissue Oxidative Stress Following Testicular Torsion and Detorsion in Rat Model

Objective-The aim of the present study was to investigate the effects of curcumin nanoparticles (CNP) on stress oxidative following experimental ischemia-reperfusion injury in the rat testes.Design-Experimental StudyAnimals- Seventy-seven healthy male Wistar ratsProcedures-The animals weighing approximately ۲۵۰ g were randomly assigned to four experimental groups (n = ۱۸): Ischemia: torsion group (created by ۷۲۰° rotation of testis on both sides for ۲ h). Ischemia/Reperfusion (I/R): torsion for ۲ h followed distortion, CNP ۱۰: received ۱۰ mg/kg IP administration of CNP ۳۰ min before surgery then remaining testes were twisted and untwisted, CNP ۲۰: received ۲۰ mg/kg IP administration of CNP ۳۰ min before surgery then remaining testicles were twisted and untwisted. Unilateral orchiectomy of left or right testicles was performed on days ۰ and ۱۲ with immediately sampling. Some twisted then untwisted testicles were remained and sampled ۶۰ days after surgery. An additional group was considered (n=۵) to be sampled without any operation as control group. The samples of testicular tissue homogenates were taken on Days ۰, ۱۲ and ۶۰, and their liquid extracts were collected and assayed for Superoxide dismutase (SOD), Malondialdehyde (MDA) and Glutathione peroxidase (GPX). The effect of treatment and day of sampling on the variables was analyzed using Two-Way ANOVA.Results- The results of the study showed an ameliorated balance of GPX, SOD and MDA following testicular torsion in this study. The CNP treated animals (with both administered doses) showed significantly improved the balance of the enzymes compared to untreated animals (p<۰.۰۰۰۱).Conclusion and clinical relevance- In conclusion, IP administration of CNP may be helpful in minimizing oxidative stress related enzymes following testicular ischemia-reperfusion in rat.

Keywords:

ischemia-reperfusion , curcumin nanoparticle , testicular torsion