Development of rapid and simultaneous detection of four major foodborne pathogens using a multiplex PCR method

Food borne diseases are an important public health problem has major impacts on human health, also affect trade and economic issues. Developing microbial cultures to detect foodborne pathogens is time-consuming and expensive. The aim of this study is to develop a multiplex (mPCR) method for the simultaneous detection of Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Salmonella enteritidis. Buffered peptone water (BPW) wasused as pre-enrichment. Simplex and multiplex PCR settings were optimized and applied to both pure co-cultures and artificially inoculated ready-to-eat food samples (falafel and chicken nugget). The four microorganisms could be detected individually and in enrichment media artificially inoculated at ۱۰۱ CFU/mL by mPCR.In conclusion, individual and combine growth of E. coli, S. enterica, S. aureus and, L. monocytogenes with low levels of contamination in the presence of food matrices such as falafel and chicken nuggets is effectively supported by BPW broth as co-culture medium before mPCR detection. The proposed protocol for pre-enrichment of E. Coli, S. Enterica, S. Aureus and, L. Monocytogenes in approximately ۳۴ hours. Compared to culture methods that require at least ۷ days. This significantly reduces analysis time, effort, and cost.

Keywords:

ready-to-eat food , multiplex PCR (mPCR) , Escherichia coli , Staphylococcus aureus , Salmonella enterica , Listeria monocytogenes