Molecular analysis of ovarian angiogenesis after treatment with ethanolic extract of SalviaOfficinalis at pre-implantation time

Phytoestrogens are nonsteroidal components of plants that bind to α and βestrogen receptors and act similar to 17-β estradiol. During recent years, more attention hasbeen focused on an angiogenic effect of S. officinalis. The effect of S.officinalis on ovarianangiogenesis molecular changes at the preimplantation time is unknown. The aim of this studyis to assess gene expression of angiogenesis in the ovarian tissue of mice after treatment withS.officinalis at preimplantation time.Method: The extract of S. officinalis leaves administered at a dose of 100 mg/kg daily for 14days to female mice.Then,Pseudopregnancy was induced and ovarian tissue samples wereremoved for molecular assessment To determine the VEGF, FLK and FLT expression, RNAextraction was performed The cDNA synthesis was accomplished by the unique quanti tectreverse transcription.