Effect of sodium nitroprusside pretreatment on the improvement of physiological traits and antioxidant enzymes in rice seedlings (Oryza Sativa L.) under salinity stress

In order to investigate the effect of sodium nitroprusside pretreatment on the improvement of physiological traits and antioxidant enzymes in rice seedlings under salinity stress, a factorial experiment was conducted in a completely randomized design with three replications at Mohaghegh Ardabil University in ۲۰۲۳. Experimental treatments included four salinity levels (۰, ۵۰, ۱۰۰, and ۱۵۰ Mm) and sodium nitroprusside in three levels (control, ۴۰ and ۸۰ µm). The results showed that salinity of growth indices, including germination percentage (GP), germination rate (GR), Seedling Length Vigor Index (SLVI) and Seedling Weight Vigor Index (SWVI), reduced, but seed priming with different levels of sodium nitroprusside, especially the level of ۸۰ µm, improved these traits. Salinity increased the Mean Germination Time (MGT), so that the highest MGT was observed at (۰.۳۷۱ days) sodium nitroprusside ۴۰ µm and salinity of ۱۵۰ mM. The Catalase (CAT) and superoxide dismutase (SOD) enzymes activity in pretreatment with sodium Nitroprusside ۸۰ µm and a salinity of ۱۵۰ mM were ۳۷ and ۴۰% higher than the control (distilled water) and without salinity, respectively. Salinity increased the activity of peroxidase enzyme, so that the lowest was observed at peroxidase enzyme (۵۸.۴۴۴ units mg-۱ protein) in the control (without salinity). Also, the peroxidase activity in pretreatment with sodium nitroprusside ۸۰ µm compared with the control (distilled water) showed an increase of about ۱۴%. The results showed that seed treatment with sodium nitroprusside ۸۰ µm improves rice seed germination and biochemical traits, it can reduce the harmful effects of salinity on some traits in rice seedlings and improve seedling growth.

Keywords:

Hydropriming , Pretreatment , Sodium Chloride , Sodium Nitroprusside , Rice , برنج , پیش‎تیمار , سدیم نیتروپرساید , کلرید سدیم , هیدروپرایمینگ