The Expression of FOXM1, PPARγ in the Placenta is affected by obesity and may lead to fetus abnormality in Rats

Background: The incidence of obesity is accompanied by the risk of many disorders such as pregnancy complications that may lead to the fetus injury. On the other hand, obesity affects the gene expression in the placenta which is the vital organ for growth and development of fetus. So we investigated the expression of two significant genes, PPAR-γ and FOXM1 which are expressed in the placenta and their expression might be affected by obesity.Material and Methods: In this study we fed one groups of female rats with normal food and 4 groups with high fat diet food for eight weeks, in order to have normal (control) and obese rats. Then all groups were mated by male rats. 18 days after the pregnancy, we remove the placenta of each rat. Then total RNA of placenta was extracted and cDNA was prepared. Then amplification of DNA was performed by TaqMan real-time PCR according to the mentioned protocol. We compare the expression of PPARγ and FOXM1 compared with GAPDH transcription.Results: The expression of both gene PPARγ and FOXM1was significantly induced in the placenta of obese rats related to nonobese rats (p value<0.05).Conclusion: Obesity makes an impression on the transcription of some genes like PPARγ and FOXM1 and in this way affects the growth and development of the fetus and even maybe lead to some disorders. So understanding this pathway might be the target for new treatment strategies for prevention and treatment of fetal disorders.

The Expression of FOXM1, PPARγ in the Placenta is affected by obesity and may lead to fetus abnormality in Rats Keywords:

Obesity , FOXM1 , PPARγ , Gene expression , Placenta