A molecular biology perspective for evaluating single-cell sequencing based on neurophysiology

The nervous system or nervous network of multicellular organisms requires communication agents and devices to coordinate the actions of the cells and organs of their bodies. The nervous system, with its specific structure and function, has been created to achieve this coordination. Neurons send nerve messages to the tissues and organs of the body, such as muscles, glands, and other neurons, and in this way communicate with them. The anatomy of the nervous system has two main parts: the central nervous system (CNS) and the peripheral nervous system (PNS). The central system is the primary command center of the body and consists of the brain and spinal cord. In fact, the peripheral nervous system consists of a network of nerves that connect the rest of the body to the CNS. These two systems work together to collect information from inside the body and from its external environment. Neurophysiology is a branch of physiology and neuroscience that studies the function of the nervous system. The main tools of basic neuroscience research include electrophysiological recordings such as patch clamp, voltage clamp, extracellular single-unit recordings, and local action potential recordings, as well as some calcium imaging, optical, and molecular biology techniques. In this article, we will discuss the integration of single-cell sequencing with various methods, such as spatial transcriptomics and electrophysiology. Finally, we will discuss the future directions of single-cell sequencing in neurophysiology.

A molecular biology perspective for evaluating single-cell sequencing based on neurophysiology Keywords:

Single cell , neurophysiology , molecular , biology , cell signaling