The Effects of Testicular Microenvironment on Proliferation of Spermatogonial Stem Cells in Azoospermia Patients
Publish place: Pathobiology Research، Vol: 19، Issue: 1
Publish Year: 1395
Type: Journal paper
Language: English
View: 19
این Paper فقط به صورت چکیده توسط دبیرخانه ارسال شده است و فایل کامل قابل دریافت نیست. برای یافتن Papers دارای فایل کامل، از بخش [جستجوی مقالات فارسی] اقدام فرمایید.
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- I'm the author of the paper
Export:
Document National Code:
JR_PRJMS-19-1_005
Index date: 18 March 2025
The Effects of Testicular Microenvironment on Proliferation of Spermatogonial Stem Cells in Azoospermia Patients abstract
Objective: This study aims to investigate the testes cultures of patients with previous histories of maturation arrest in spermatogenesis and find the appropriate methods to overcome this problem. Methods: We divided spermatogonial stem cells (SSCs) isolated from testes biopsies into 3 groups: 1) culture of SSCs without feeder layer; 2) co-culture of SSCs with patient-derived Sertoli cells; and 3) co-culture of SSCs on Sertoli cell feeder layer derived from healthy donors. We calculated the numbers and diameters of stem cell-derived colonies and the percentage of cell viability in the different groups. The presence of SSCs at different culture times was determined by immunochemistry, alkaline phosphatase, and xenotransplantation of SSCs into an azoospermic mouse model. Results: The microenvironment of the feeder layer derived from the patient’s own Sertoli cells produced numerous (36.1±4) large colonies (213.2±17 µm) after 3 weeks of culture. However, the ratio of germ cell-specific expressions of Stra8 (2.3) and Vasa (2.2) was more than the pluripotency gene, Nanog (0.45) in SSCs cultured on the Sertoli cell layer of a healthy person. After xenotransplantation of human SSCs into the testis of an azoospermic mouse model, we observed that the cells grow on basement membrane of seminiferous tubules, which confirmed their nature. Conclusion: SSCs could be co-cultured with Sertoli cells derived from healthy donors in order to overcome the arrest of spermatogenesis observed in the co-culture of SSCs with patient-derived Sertoli cells. The results of the present study indicated that spermatogenesis could possibly be resumed in cancer patients previously treated by chemotherapy and∕or radiotherapy.
The Effects of Testicular Microenvironment on Proliferation of Spermatogonial Stem Cells in Azoospermia Patients Keywords:
Sertoli cells , testis microenvironment , Spermatogonial stem cells , ریز محیط بیضه , سلول های بنیادی اسپرماتوگونی , سلول های سرتولی
The Effects of Testicular Microenvironment on Proliferation of Spermatogonial Stem Cells in Azoospermia Patients authors
طوبا میرزاپور
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
ابوالفضل بایرامی
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :