Study of Protective Effect of Nigella Sativa Nanocapsules on Tissue Pathology and IL-10 levels in Lung Tissues Exposed to NNK

Aims: It is assumed that ketone derived from tobacco (NNK) which is the most important carcinogen in tobacco modulates alveolar macrophage mediator production, such as anti-inflammatory cytokine IL-10. The aims of this study were to investigate the effect of a Nigella sativa on tissue pathology and IL-10 levels in lung tissues exposed to NNK Materials & Methods: In this experimental study, 46 Wistar rats of supplement, supplement+NNK, NNK, solvent and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of 12.5 mg per kg body weight. Supplemented groups also consumed Nigella sativa Nanocapsules for 12 weeks. Levels of IL-10 in homogenized lung tissue were measured by ELISA. To analyze the data; ANOVA and Tukey test were used at a significance level of p≤005. Findings: Exposure to NNK increased levels of IL-10 compared with the solvent group, although not statistically significant (p≥005). Meanwhile, a period of consumption of Nigella sativa Nanocapsules significantly increased levels of IL-10 in NNK + supplement  and the supplement groups compared to NNK group (p=0.038, p=0.002; respectively). In addition, the effect of Nigella sativa Nanocapsules on IL-10 levels of lung tissue was shown that the levels of this variable were significantly higher than the solvent group (p=0.001). Conclusion: Generally it could be confirmed that consumption of Nigella sativa Nanocapsules plays an important role in the inhibition of inflammation induced by NNK via Increase of IL-10 activity. Aims: It is assumed that ketone derived from tobacco (NNK) which is the most important carcinogen in tobacco modulates alveolar macrophage mediator production, such as anti-inflammatory cytokine IL-10. The aims of this study were to investigate the effect of a Nigella sativa on tissue pathology and IL-10 levels in lung tissues exposed to NNK Materials & Methods: In this experimental study, 46 Wistar rats of supplement, supplement+NNK, NNK, solvent and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of 12.5 mg per kg body weight. Supplemented groups also consumed Nigella sativa Nanocapsules for 12 weeks. Levels of IL-10 in homogenized lung tissue were measured by ELISA. To analyze the data; ANOVA and Tukey test were used at a significance level of p≤005. Findings: Exposure to NNK increased levels of IL-10 compared with the solvent group, although not statistically significant (p≥005). Meanwhile, a period of consumption of Nigella sativa Nanocapsules significantly increased levels of IL-10 in NNK + supplement  and the supplement groups compared to NNK group (p=0.038, p=0.002; respectively). In addition, the effect of Nigella sativa Nanocapsules on IL-10 levels of lung tissue was shown that the levels of this variable were significantly higher than the solvent group (p=0.001). Conclusion: Generally it could be confirmed that consumption of Nigella sativa Nanocapsules plays an important role in the inhibition of inflammation induced by NNK via Increase of IL-10 activity.

Study of Protective Effect of Nigella Sativa Nanocapsules on Tissue Pathology and IL-10 levels in Lung Tissues Exposed to NNK Keywords:

Nigella sativa , Nanocapsules , IL-10 , NNK , Histologic Structure , نانوکپسول سیاه دانه , اینترلوکین 10 , NNK , ساختار هیستولوژیک