Investigation of the partitioning behavior of plasmid DNA as a nanobioparticle in PEG300/salt aqueous two phase system
Publish place: 11th National Iranian Chemical Engineering Congress
Publish Year: 1385
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NICEC11_667
تاریخ نمایه سازی: 4 اردیبهشت 1386
Abstract:
Plasmid DNA (pDNA) based gene therapy belongs to the category of non-viral systems. Generally, their production is considered more scalable, the process is easier to control, monitor and quality control testing is considered easier than that for viral vectors. Since downstream processing techniques for the recovery of nanobioparticles face to problems other than those encountered during the recovery of biomolecules such as peptides and proteins, thus downstream process routes that are currently in use for bioparticles have to be re-designed for such nanoparticles. Aqueous two phase system (ATPS) is one of the processes that are used for this purpose. Partitioning of plasmid DNA in ATPS has been shown to provide a powerful method for separation and purification mixture of nanobioparticles. In this paper, Polymer-salt system have been studying and the partition behavior of pDNA in aqueous two phase systems of polyethylene glycol 300 – potassium phosphate at different temperatures and lysate concentrations were investigated. The volume ratio and recovery of pDNA in top, bottom and interface were measured. The results showed that lysate concentration from 10 to 50 mg/ml as well as temperature from 5 to 45°C have a significant effect on pDNA recovery.
Keywords:
Aqueous two phase systems , Nanobioparticle , Plasmid DNA , Polyethylene glycol , Potassium phosphate
Authors
Tabandeh
Industrial Biotechnology Dept., National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Khavarpour
Nanobiotechnology Lab., Faculty of Chemical Engineering, Mazandaran University, Babol, Iran.
Jahanshahi
Nanobiotechnology Lab., Faculty of Chemical Engineering, Mazandaran University, Babol, Iran.
Khodabandeh
Industrial Biotechnology Dept., National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
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