Production of scFv Monocolonal Antibody Versus Specific Marker Of Prostate Cancer
Publish place: 8th national biotechnology congress of I.R. Iran & 4th National Conference on biosecurity
Publish Year: 1392
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NBCI08_0934
تاریخ نمایه سازی: 29 شهریور 1394
Abstract:
The researches in the field of radioimunotherapy and chemoimunotherapy are based on monoclonal antibody production and demonstrate that these methods are effective in inhibiting the growth of prostate tumor. Today hybridoma and phage display methods are used for production of monoclonal antibodies. Phage display method is widely used for monoclonal antibody production because of its inexpensivity. One of the methods used in isolation of monoclonal antibodies is use of artificial antibody libraries that include more than 100 million different scFv fragments coloned in ampicillin resistance phagmid vector and have transformed into E. coli cell ( scFv fragments contain single polypeptide with VL and VH domains that have been connected to each other’s with flexible connector (serine-glycine)) scFv fragments have various merits, for example they have small size, high perfusion, high yield production and non-immunogenicity (because of Fc fragment elimination), thus they can be used for therapeutic purposes .
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Authors
Javad Rezaei
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
Masoumeh RajabiBazl
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
Seyedlatif Mousavi
Department of Biology, Faculty of Basic Science, Shahed University, Tehran, Iran
Gholamreza Rafiei Dehbidi
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
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