Solid Phase Extraction and Determination of Bentazon in Surface Water by Molecularly Imprinted Polymers

In this paper, a highly selective sample cleanup procedure combining molecular imprinting and solid phase extraction (MI-SPE) was developed for the isolation of bentazon in surface water. The molecularly imprinted polymer (MIP) was prepared using bentazon as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer. The bentazon imprinted polymer was used as selective sorbent for the solid-phase extraction of bentazon from surface water. An off-line MI-SPE method followed by high-performance liquid chromatography was also established. In order to evaluate the applicability of the MIP for separation and determination of bentazon by HPLC, general parameters for SPE including the amount of loading solvents, washing solution and eluent and pH of the sample were optimized following a step by step approach. It was shown that recoveries up to approximately %49 from spiked surface water samples could be obtained. It was demonstrated that the proposed MI-SPE-HPLC method could be applied to direct determination of bentazon in surface water.

Keywords:

Molecular imprinting , Solid phase extraction , Bentazon