Investigarion of infusion modulates gcl and gpx4 gene expression of (Sambucus ebulus L) extracts in IRA (Anzali port)

Glutamate-cysteine ligase and glutathione peroxidase 4 are enzymes involved in cellular antioxidant defense. Biologically active compounds, such as polyphenols, may modulate their gene expression. Sambucus ebulus L. is a plant, which fruit infusions exhibits high antioxidant activity, due to its high polyphenol content. We analyzed the effect of Sambucus ebulus fruit aqueous infusion on glutamatecysteine ligase catalytic subunit and glutathione peroxidase 4 gene expression levels in the presence and in the absence of oxidizing agent tert-butyl hydroperoxide in a 3T3-L1 preadipocyte cell culture model. Gene expression was analyzed by Real-Time qPCR, and relative gene expression levels were calculated using 2–++Ct method. Viability of treated preadipocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid reduction assay. In the range of 1.25% to 12.5% content of the infusion in the culture medium cell viability was not affected signifi cantly. Glutamate-cysteine ligase mRNA levels were increased signifi cantly (p < 0.05) after treatment. Pretreatment decreased tert-butyl hydroperoxide-induced gene expression of glutamate-cysteine ligase (p < 0.05) and of glutathione peroxidase 4 (p <0.05). These data suggest that Sambucus fruit infusion exhibits strong antioxidant activity by modulating glutamate-cysteineligase and glutathione peroxidase 4 gene expression. The study provides fi rst scientifi c data about the antioxidant activity of Sambucus ebulus fruit infusion in a cell culture model.

Keywords:

antioxidant , glutamate-cysteine ligase , glutathione peroxidase 4 , Sambucus ebulus , 3T3-L1cells Abbreviations: GCLc – glutamate-cysteine ligase catalytic subunit ,