Detection of salmonella in raw poultry meat by PCR and traditional culture method

Contaminated poultry meat has been identified as one of the principal foodborne sources of salmonella.Traditional culture method for detection of salmonella requires 4-6 days to processing samples. PCR has became the potential of a powerful alternative in microbiological diagnostics due to its rapidity and accuracy. In this study a survey was carried out to determine the prevalence of salmonella in raw broiler carcasses. A total of 60 neck skin swab samples were taken from broiler carcasses after the chilling stage of processing. The presence of salmonella was assessed by traditional culture method and confirmed by serotyping and PCR amplificaton of invA gene. Incorporation of a routin PCR test in conjunction with standard culture,could be effective in providing a more accurate profile of the prevalence of this pathogen in broiler carcasses.

Keywords:

Poultry , Salmonella , PCR , Traditional method