Salinity response pattern of glyoxalase I gene from halophyte plant Aeluropus littoralis
Publish Year: 1394
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICSDA02_217
تاریخ نمایه سازی: 9 مرداد 1395
Abstract:
Aeluropus littoralis (Gouan) Parlatore is a rhizomatous perennial halophyte monocotyledonous that can withstand environmental stresses such as salinity. In this study, the relative expression of glyoxalase I (GLY I) gene, as one of the main factors causing stress tolerance, were investigated in A.littoralis under salinity and recovery conditions by Real-time PCR. A.littoralis seedlings towards this purpose were subjected gradually to salt stress using 600 mM NaCl. Plant samples, were prepared, separately from the shoot and root tissues after 6 h, 24 h and 1 week behind the salt treatment, as well as 6h, 24h and 1 week after stress removing. Real-Time PCR was performed, in order to the GLY I expression level assessment. The results suggested significant increases of the GLY I expression levels, that were more remarkable among salt stressed treatments compared to the recovered samples. Maximum GLY I activities were observed after 6 and 24h salt stress that were 6 and 3.5 fold higher, respectively, compared to the control. Also these increases were more greater in shoot tissues than root ones. Overexpression of GLY I under NaCl stress, in fact was considered as a salinity tolerance factor in halophyte A.littoralis.
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Authors
Sahar faraji
MSc Student of plant breeding,
Hamid Najafi Zarrini
Sari Agricultural Sciences and Natural Resources University
Seyyed Hamid Reza Hashemi
Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University
Gholam Ali Ranjbar
Sari Agricultural Sciences and Natural Resources University
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