Antibacterial Activity of the Hydro-Alcoholic Extract of Juglans regia L. Stem Bark on Human Bacterial Infection

Aims Bovine mastitis continues to be the most costly disease to the dairy farmers. Itdominates in Iran as one of the most prevalent diseases in dairy cattle among the dairy farms.Mastitis treatment with antibiotics leads to the development of antibiotic resistant strainsand consumer health problem.This study was performed for the first time to analyze in vitroeffects of hydro-alcoholic extract of Juglans regia L. stem bark on 6 mastitis pathogens.Materials & Methods the susceptibility of 6 strains of bacteria (Staphylococcus aureus,Escherichia coli, Klebsiella pneumoniae, Streptococcus spp., Pasteurella multocida andMannheimia haemolytica) were analyzed against hydro-alcoholic extract of Juglans regiaL. stem bark with minimum inhibitory concentration (MIC) and minimum bactericidalconcentration (MBC) methods.Findings Hydro-alcoholic extract did not have antibacterial effects on E. coli and K.pneumoniae. Minimum inhibitory concentration for S. aureus, P. multocida, M. haemolyticaand Streptococcus spp. was 62.5mg/ml of hydro-alcoholic extract. There was not anysignificant response with concentrations below 100mg/disc on S. aureus, Streptococcusspecies, P. multocida and M. haemolytica. Minimum bactericidal concentration of this extractwas 100mg/ml in all isolates.Conclusion Juglans regia L. have some antibacterial effects on S. aureus, P. multocida, M.haemolytica and Streptococcus species.

Keywords:

Mastitis , Bovine , Disk Diffusion Antimicrobial Tests , Juglans