Application of different enzyme in detergents

Alkaline crude enzymes from the viscera of the Tunisian barbel (Barbus callensis) were extracted and characterized. Proteolytic crude extract from barbel viscera was active and stable in alkaline solution. The crude alkaline proteases showed stability towards various surfactants, bleach agents and compatibility with some commercial detergents. Alkaline proteases from the viscera of the barbel were tested in chicken feather-degradation and showed important feather degrading activity. The lipase showed optima activity at pH 8.5 and 20 ◦C. The lipase activity was activated by metal ions, such as Ca2+ and Mn2+, and surfactants, such as Tween 80, Tween 20, sodium dodecyl benzene sulfonate and urea. Oxidising agents, such as H2O2 and NaClO, were found to have little effect on the activity of the lipase, and most organic solvents can enhance the activity of the lipase. The optimum temperature for enzyme activity was 60 ◦C, and the activity and stability of trypsin was highly dependent on the presence of calcium ion. At 60 ◦C, Ca2+ (5 mM) stimulated the protease activity by 220%. The trypsin kinetic constants, Km and kcat, were 0.312 mM and 2.03 s−1. The enzyme showed high stability towards non-ionic surfactants and oxidizing agent. In addition, the enzyme showed excellent stability and compatibility with some commercial solid and liquid detergents.

Keywords:

enzyme , detergent , protease , Lipase