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Assessing the Frequency and AntibioticSusceptibility Pattern of Isolated Bacteria fromSepticemic Hemodialysis Patients

فصلنامه تمرکز بر علوم، دوره: 1، شماره: 1
Year: 1394
COI: JR_FOCS-1-1_003
Language: EnglishView: 399
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Elmira Gheytanchi Mashini - Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran
Romina Karimzadeh Ghassab - MSc of Clinical Biochemistry, Tehran, Iran


Introduction: Septicemia is one of the main causes of morbidity and mortality worldwidethat increases the hospitalization time and also raises the cost for patients. The currentstudy aimed to evaluate the frequency and antimicrobial susceptibility profiles of bloodculture isolates from the hemodialysis patients referred to Hasheminejad Hospital inTehran, Iran.Methods: In this retrospective cross-sectional study the records of 1090 patients whoundergone hemodialysis in Hasheminejad Hospital Urinary Tract and Kidney Centerbetween 2012 and 2013 were evaluated. At least two Blood samples from each patientswere collected under sterile conditions and was injected into blood culture bottles. After 1,3, 5 and 7 days, samples were cultured in sheep blood agar (BA), chocolate agar and eosinmethylene blue agar (EMB). Disc diffusion on Muller Hinton Agar (HIMEDIA, India)was performed to define the susceptibility. Spss software version 20 was used to analyzethe data.Results: From 1090 patients 186 subjects had positive blood culture from them 121 weremale and 65 were female. The most frequent isolated species are as follow respectively:coagulase positive Staphylococcus 68 (37%), Escherichia coli 47 (26%), Pseudomonasaeruginosa 25 (14%), Streptococcus Group D 22 (12%), Coagulase-negative Staphylococcus13 (7%), Streptococcus group A 4 (2%), Klebsiella 2 (1%), and Bacillus 1 (1%). Gramnegative bacteria were mostly sensitive to nitrofurantoin, amikacin, and ciprofloxacin.In addition, gram positive bacteria were mostly sensitive to vancomycin, amikacin,cefotaxime, ciprofloxacin, imidazole, colistin, erythromycin, and oflatoxin.Conclusion: The result of the current study determined the most prevalent bacteriathat are responsible for septicemia in Tehran, Iran, and the most effective antimicrobialsfor treatment of septicemia in this area which could help physicians to select a properantibiotics for initial antimicrobial therapy


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Gheytanchi Mashini, Elmira and Karimzadeh Ghassab, Romina,1394,Assessing the Frequency and AntibioticSusceptibility Pattern of Isolated Bacteria fromSepticemic Hemodialysis Patients,

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Type of center: علوم پزشکی
Paper count: 4,517
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